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Agrobacterium mediated gene delivery

Published by Admin in Geneneral Botany
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For more information, log on to-http://shomusbiology.weebly.com/Download the study materials here-http://shomusbiology.weebly.com/bio-materials.htmlTransfer of T-DNA into the plant cellA: Agrobacterium tumefaciensB: Agrobacterium genomeC: Ti Plasmid : a: T-DNA , b: Vir genes , c: Replication origin , d: Opines catabolism genesD: Plant cellE: MitochondriaF: ChloroplastG: NucleusThe T-DNA must be cut out of the circular plasmid. A VirD1/D2 complex nicks the DNA at the left and right border sequences. The VirD2 protein is covalently attached to the 5' end. VirD2 contains a motif that leads to the nucleoprotein complex being targeted to the type IV secretion system (T4SS).In the cytoplasm of the recipient cell, the T-DNA complex becomes coated with VirE2 proteins, which are exported through the T4SS independently from the T-DNA complex. Nuclear localization signals, or NLS, located on the VirE2 and VirD2 are recognised by the importin alpha protein, which then associates with importin beta and the nuclear pore complex to transfer the T-DNA into the nucleus. VIP1 also appears to be an important protein in the process, possibly acting as an adapter to bring the VirE2 to the importin. Once inside the nucleus, VIP2 may target the T-DNA to areas of chromatin that are being actively transcribed, so that the T-DNA can integrate into the host genome. Source of the article published in description is Wikipedia. I am sharing their material. Copyright by original content developers of Wikipedia.Link- http://en.wikipedia.org/wiki/Main_Page

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